Unknown A: Acid Fast and Endospore Staining

    Today in class, we preformed two stains. The acid fast and the endospore stain. The purpose of these test is as their names suggest: to see if the cell wall is acid fast and to see if the bacteria possesses endospores.
    We only have pictures for the acid fast stain, but the procedure was the same for both.
1. Fix bacteria to the slide
2. Put fixed slide over steaming water
3. Place paper on top of the slide
4. Douse with the appropriate dye
5. Do this for the appropriate amount of time then remove and cool
6. Rinse excess stain off and cover with another stain
7. Rinse this stain off and blot with bibulous paper
8. Exam under microscope! :)

 Ari putting stain on our sample

The finished product

Conclusion: Not Acid Fast

Even though we don't have pictures for the endospore stain... we do have one picture of our mess up :( We had a little spill and my lab jacket suffered from our mistake! We were definitely more careful from then on! :)

Until next time then,

JTA and ART