Glucose, Lactose, Sucrose and TSI Enzyme Presence

10/15

The "Before" picture of the tubes are from left to right: Sucrose, Glucose, Lactose and TSA

All tests were to determine if our bacteria could ferment a particular carbohydrate, in this case Lactose, Sucrose and Glucose.

Glucose: Positive

It changed color to yellow indicating acid and it had a bubble indicating gas.

See the bubble?

Lactose: Positive

It changed color to yellow indicating acid and it had a bubble indicating gas.

Pretty yellow no?

Sucrose: Negative

Sadly, this test was negatory! But, at least it was still a pretty color! :) The VERY bright pink indicates no breaking down of sucrose! 

TSI: Positive

To see which one, glucose or sucrose, that the bacteria really eats.

Our results were: Acid slant/alkaline but, with Hydrogen disulphide gas (Black at the bottom).

Until next time then! 

JTA and ART

:)