DRUGS:
Novobiocin - it did not work it is a nucleic acid synthesis inhibitor
Erythromycin - nothing happened, this is translation ribosome inhibitor
Aminoglycoside - (this particular antibiotic binds the 30s ribosome and allows it to misread the RNA) this seemed to work best on all gram negative bacteria, it worked on ours 2cm
Tetracycline - this breaks the attachment of tRNA, this worked well it was 4cm
Penicillin - this breaks down the cell wall. This worked very well with our bacteria, it was 4cm
Cefoxtin - this also breaks the cell wall, and it did a good job on ours it was 4cm too.
Anaerobic Chamber:
The blue strip of paper indicates that oxygen is present. It was white previously indicating that no oxygen was present. What we did in this experiment was suck the oxygen out of the chamber. This would test if our bacteria could grow in side. Our bacteria hardly grew, meaning it does need oxygen, but it can do without it just it will not thrive.
Yogurt:
We had previously mixed some yogurt into cold milk and then into milk that had been heated. Our result was that there was yogurt produced in both, but the milk that had been heated was less sour than the one that had not been. Maybe this was because the temperature killed the bacteria that secrete the sourness as a taste? We are not really sure.
Heated
Not Heated
Not Heated
RETESTING
We retested our bacteria for the Lactose test and the urease test. Our results were lactose negative, urease positive:
here is a picture of the urease because it was positive, it had a extreme change in color! This surprised us, because our last test result was negative. We assume that we must not have inoculated it or something because how else could it have produced such a different result? But any how, after following the flow chart this led us to the conclusion that our unknown bacteria was Proteus Vulgaris! This is a ear infection bacteria :)
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