Lots of Inoculations!

These are the tests we preformed/ inoculated we await the results in our upcoming lab. 

1. Blood Agar Plate

2. Eosin Methylene Blue Agar (EMB)

3. Mannitol Salt Agar

4. MacConkey Agar

5. Phenylethyl Alcohol AGAR (PEA)

Glucose, Lactose, Sucrose and TSI Enzyme Presence

10/15

The "Before" picture of the tubes are from left to right: Sucrose, Glucose, Lactose and TSA

All tests were to determine if our bacteria could ferment a particular carbohydrate, in this case Lactose, Sucrose and Glucose.

Glucose: Positive

It changed color to yellow indicating acid and it had a bubble indicating gas.

See the bubble?

Lactose: Positive

It changed color to yellow indicating acid and it had a bubble indicating gas.

Pretty yellow no?

Sucrose: Negative

Sadly, this test was negatory! But, at least it was still a pretty color! :) The VERY bright pink indicates no breaking down of sucrose! 

TSI: Positive

To see which one, glucose or sucrose, that the bacteria really eats.

Our results were: Acid slant/alkaline but, with Hydrogen disulphide gas (Black at the bottom).

Until next time then! 

JTA and ART

:)

 

Indole, Urea, Nitrate, Methyl Red, Citrate, TSA (TBA)

10/17
Indolde Test: Positive
This indicates that our bacteria can turn amino acid tryptophan into indole and pyruvic acid.
Positive is indicated by a pretty red band at the tops! :)

Urea Test: Negative
This indicates there is no degradation of urea.
No change in color shows this. The one on the left was our inoculated tube and the one on the right is an uninoculated tube.

Nitrate Test: Positive
Determine if the bacteria can reduce the nitrate to nitrate ions or nitrate gas. Presence of dark red color indicates nitrate ions present.

Methyl Red Test: 

Part A: Positive

Part B: Negative

A: Determines the ability to ferment glucose via mixed acid fermentation

This was positive because because the color turned red. 

B: Determines if there is butane diol fermentation:

This was negative as indicated because there was no change in color. We did not take a picture... because you should be able to use your imagination by now. JUST KIDDING!!!

Citrate test: Negative

Determine the ability of the bacteria to digest citrate. Our color stayed green indicating a negative test result.

TSA (TBA): Negative

Aka Oxidase test:

Yellow color indicates no presence of Cytochrole Oxidase

Lots of Tests Day! :)

Today we did many tests to test for certain enzymes that our bacteria had. 

1. Motility Test: To see if our bacteria is motile. We had to inoculate a motility test tube and this was the result: YES MOTILE! :)

 2. Litmus broth milk tests: Result Negative!

 3. Here is pictured our gelatin test and our litmus test. The gelatin was positive!

 

 3. Broth Drop bacteria technique

 We tried multiple times and this is what we got each time... all different so we decided to post them all anyway.

 3. Skim milk plate: result negative

 4. Starch Plate Result: Negative

Next Time... Checking out whether it has sugar digesting enzymes

Fun Virus Day

Today is a short post, we had smeared our bacteria on a agar streak plate. Dr. P gave us a virus and told us to write our name in the bacteria with the virus. He told us to look at it when we came back next week and the bacteria would have been eaten away by the virus. This was our result:

I don't know if you can see it, but if you look for a little line down the middle that is where the virus "ate" the bacteria!

Unknown A: Acid Fast and Endospore Staining

    Today in class, we preformed two stains. The acid fast and the endospore stain. The purpose of these test is as their names suggest: to see if the cell wall is acid fast and to see if the bacteria possesses endospores.
    We only have pictures for the acid fast stain, but the procedure was the same for both.
1. Fix bacteria to the slide
2. Put fixed slide over steaming water
3. Place paper on top of the slide
4. Douse with the appropriate dye
5. Do this for the appropriate amount of time then remove and cool
6. Rinse excess stain off and cover with another stain
7. Rinse this stain off and blot with bibulous paper
8. Exam under microscope! :)

 Ari putting stain on our sample

The finished product

Conclusion: Not Acid Fast

Even though we don't have pictures for the endospore stain... we do have one picture of our mess up :( We had a little spill and my lab jacket suffered from our mistake! We were definitely more careful from then on! :)

Until next time then,

JTA and ART